4th International Giardia Conference and first combined
Giardia/Cryptosporidium meeting in Amsterdam

20-24 September 2004

Scientific Programme

You can download a pdf with the program at the bottom of this page 


Monday 20th September


08.30          Registration


09.30          Welcome -  L.M. Kortbeek (The Netherlands) and R.C.A. Thompson (Australia)


09.45          Opening Lecture - Leeuwenhoek’s revelations

                    B.J. Ford (United Kingdom)


10.45          Break


                    Taxonomy & Nomenclature

                    Chair: R.C.A. Thompson (Australia)


11.15               Giardia: Genetic change and constancy

                    R.A. Adam (USA)


11.45          Developments in taxonomy

                    M. Tibayrenc (France)


12.15          Discussion


12.30          Lunch


                    Molecular typing Giardia / Cryptosporidium

                    Chair: R.C.A. Thompson (Australia)


13.30          Molecular epidemiology of human infections caused by Cryptosporidium and Giardia

                    S.M. Caccio (Italy)


14.00          Giardia and Cryptosporidium of wild and zoo mammals

                    A. Appelbee (Canada)


14.30               Molecular identification of human Giardia strains from The Netherlands and phylogenetic analysis using 18S-RDNA and GDH sequences

J.G.M. van der Giessen (The Netherlands)


14.50          Development of an RT-PCR method for detection and differentiation of Cryptosporidium species of public health concern

                    C.M. Read (Australia)


15.10          Detection and genotyping of Giardia duodenalis from human and animal isolates in Italy

                    F. Berrilli (Italy)


15.30          Break


16.00          Poster presentation


Tuesday 21nd  September


                    New technologies

                    Chair: A. Appelbee (Canada) and T.G. Mank (The Netherlands)


09.00          Emerging technologies for the detection and genetic characterisation of Cryptosporidium and Giardia

                    P. Monis (Australia)


09.30          Molecular techniques for genotyping Cryptosporidium and Giardia isolates

                    S. Caccio (Italy)


10.00          Development of a novel genetic fingerprinting method for Giardia lamblia

                    R. Jansen (The Netherlands)


10.15          A novel genotyping method for Cryptosporidium spp.: current status of mixed infection with different genotypes

                    M. Tokoro (Japan)


10.30          Break


                    Molecular typing Giardia and Cryptosporidium continued

                    Chair: A. Appelbee (Canada) and T.G. Mank (The Netherlands)


11.00         Genetic Characterization by random amplified polymorphic DNA analysis(RAPD) of 18

                    isolates of Giardia lamblia obtained from day care children. Description of a genetic

                   marker of pathogenicity

                    L. de los Angeles Pelayo Duran (Cuba)


11.20          Cryptosporidiosis in Zambian patients with diarrhoea: Molecular and clinical


A. Ayoub (United Kingdom)


11.40          Molecular typing of Crytosporidium and Giardia in Danish cattle and pig herds

                    R.B. Langkjaer (Denmark)


12.00          Database development of Giardia and Cryptosporidium for phylogenetic analysis

                    A. de Vries (The Netherlands)


12.30          Lunch and poster presentations


                    Zoonotic issues

                    Chair: J.G.M. van der Giessen (The Netherlands) and B.R. Dixon (Canada)


13.45         The Zoonotic Significance of Giardia and Cryptosporidium

                    R.C.A. Thompson (Australia)


14.15              Genetic comparison between Giardia isolates of human and animal origin by PCR-RFLP in Iran: Evidence of zoonotic transmission

M. Rezaeian (Iran)


14.35              Giardia and Cryptosporidium in the intestinal contents of ringed seals and bearded seals in Nunavik, Quebec, Canada

B.R. Dixon (Canada)


15.00      Parallel workshops


Workshop Molecular typing

Chair: L.M. Kortbeek (The Netherlands) and T.G. Mank (The Netherlands)


-        Taxonomy

-        A/B Dichotomy and Pathogenicity

-        Genotyping of environmental Cryptosporidium

Workshop Other waterborne protozoa

Chair: P. Karanis (Germany)

Waterborne transmission of protozoan parasites: a review of worldwide outbreaks

P. Karanis (Germany)


Combined flocculation with nested-pcr for the detection of Toxoplasma gondii in water

P. Karanis (Germany)

Isolation and identification of free-living amoebae from drinking water sources collected in western Japan.
A. Kimura (Japan)

How do Acanthamoeba cross the blood-brain barrier to produce granulomatous amoebic encephalitis
Naveed Ahmed Khan (United Kingdom)

Balamuthia mandrillaris: an increasingly important pathogen
Naveed Ahmed Khan (United Kingdom)

Clinical and environmental distribution of Acanthamoeba spp. in Iran
Amir Hossein Maghsood (Iran)

Detection of Cyclospora and Isospora from diarrheal feces and tap water collected from Nepal and Lao PDR
K. Kimura (Japan)


Wednesday 22nd September


                    Control of infection

                    Chair: T. Paget (United Kingdom)


09.00          Control of Giardia and Cryptosporidium infections in humans



09.30          Control of Giardia and Cryptosporidium infections in animals

                    M. Olson (Canada)         


10.00          Prevention and Control of Cryptosporidium and Giardia in the Environment

                    R. Fayer (USA)


10.30          Break


                    Control of infection continued    

                    Chair: T. Paget (United Kingdom)


11.00          Characterization of S-adenosyl-L-homocysteine hydrolase gene from Cryptosporidium: assessment of drug candidates against cryptosporidiosis

                    M. Tokoro (Japan)


11.20          Genotype-linked metronidazole sensitivity of clinical Giardia duodenalis isolates

                    L. Favennec (France)


11.40          A high level of albendazole resistance in vitro involves multidrug resistance in Giardia

                    G. Ortega – Pierres (Mexico)


12.00          Lunch with poster presentations



                    Chair: L.M. Kortbeek (The Netherlands)


13.30          Diagnostic possibilities in human infections

                    T.G. Mank (The Netherlands)


14.00          Validation of flow cytometry for the detection of Giardia and Cryptosporidium spp. in bovine fecal samples: preliminary results

                    F. Uehlinger (Canada)


14.20               Multiplex detection of Entamoeba histolytica, Giardia lamblia and Cryptosporidium parvum in faecal samples using real-time PCR

J. Verweij (The Netherlands)


14.40          Detection of Giardia lamblia in stool samples at people in collective accommodation

N. Miladinovic-Tasic (Serbia and Montenegro)


                    Clinical presentations

                    Chair: L.M. Kortbeek (The Netherlands)


15.00          Giardiasis, clinical presentation and therapy           

                    J.J.M. Tolboom (The Netherlands)


15.30          Cryptosporidiosis clinical presentation and therapy

                    V. Kartashev (Russia)


16.00          Break


                    Clinical presentations continued

                    Chair: L.M. Kortbeek (The Netherlands)


16.30         The prevalence and correlation of Giardiasis with the nutritional status of primary school students in Iran - Esfahan

                    M. Azami (Iran)


16.50          Giardia lamblia: different genotypes, different symptoms?

                    T.G. Mank (The Netherlands)


17.10          Cryptosporidium infection in Kala azar patients in Sudan

                    G. Elfadil (Sudan)


19.30          Conference dinner


Thursday 23rd September



Chair: L.M. Kortbeek (The Netherlands)


09.00          Epidemiology of Cryptosporidium and Giardia

                    P.R. Hunter (United Kingdom)


09.30          Giardia and Crypto; the Dutch situation

                    L.M. Kortbeek (The Netherlands) and T.G. Mank (The Netherlands)


10.00          Investigating the public health significance of Cryptosporidium in the environment

                    G. Robinson (United Kingdom)


10.20          Estimation of characteristics of four diagnostic tests and prevalence of Cryptosporidium spp. oocysts excretion by a Bayesian approach on two Irish farms

                    V. De Waele (United Kingdom)

10.40          Giardia cysts and Cryptosporidium oocysts in a surface watersource for human consumption in Viçosa, Minas Gerais State, Brazil

                    P.D. Bevilacqua (Brazil)


11.00          Break


                    Epidemiology continued

                    Chair: L.M. Kortbeek (The Netherlands)


11.30          Prevalence of Cryptosporidium infection of farm animals in Kerman, Iran

                    M. Fasihi Harandi (Iran)


11.50         Prevalence of intestinal protozoal parasites in relation to HIV serostatus and complains of chronic diarrhoea in Blantyre, Malawi

                    L. van Lieshout (The Netherlands)


12.10          Lunch with poster presentations


Thursday afternoon: two parallel sessions


                    Cell Biology – parallel session 1

                    Chair: T.G. Mank (The Netherlands) and H. Smith (United Kingdom)


13.30          Encystment as a target for drug design

                    E.L. Jarroll (USA)


14.00          The protein secretory pathways and mitochondrial remnants in Giardia and Cryptosporidium

                    P. Köhler (Switzerland)


14.30          Apoptosis in Giardia: still more questions than answers

                    N. Kitchener


14.50          Modulation of host polyamine biosynthetic enzymes by Cryptosporidium parvum

                    N. Yarlett (USA)


15.10          Advances in the Developmental Biology of Cryptosporidium

                    N. Hijjawi (Australia)


15.30          Break


                    Pathology – parallel session 1   

                    Chair: T. Paget (United Kingdom)


16.00          Pathogenesis of Giardia and Cryptosporidium

                    A.G. Buret (Canada)


16.30          Altered gene expression during Giardia- host cell interactions

                    K. Roxström- Lindquist (Sweden)


16.50          Gut Cryptosporidium parvum (genotype II) infection of unweaned immunocompetent rats results in long-terme jejunal hypersensitivity to distension

                    R. Marion (France)


               Drinking water treatment 1 – parallel session 2

                    Chair: G.J. Medema (The Netherlands)


13.30          Cryptosporidium and Giardia in drinking water in Amsterdam

                    Y. Dullemont     


14.00          Optimization of a purification plant for the detection of Cryptosporidium and Giardia: A Rand Water Experience

                    M. Grundlingh (South Africa)


14.20          The effects of water treatment processes on Cryptosporidium infectivity and disinfection

                    P. Monis (Australia)


14.40          Potential Cryptosporidium oocyst surrogates for sand filtration and the importance of their surface properties

                    J. Chung (Australia)


15.00          Break


                    Swimming pools and surface water – parallel session 2  

                    Chair: P. Hunter (United Kingdom)


15.30          Cryptosporidium and Giardia in swimming pools in the Netherlands

                    C. Schets (The Netherlands)                        


15.50          Assessing geospatial and geotemporal contamination of a watershed with Cryptosporidium and Giardia using molecular forensic profiling

                    N.F. Neumann (Canada)


16.10          Quantifying the impact of runoff events on Cryptosporidium entering drinking source waters

                    R. Signor (Australia)                      


                    Wastewater – parallel session 2

                    Chair: P. Karanis (Germany)


16.30          Effects of sewage effluent treatment processes on the viability of Giardia muris cysts

                    D. Bowman (USA)


16.50          Cryptosporidium oocysts and Giardia cysts in wastewater

                    L. Weaver (United Kingdom)


17.10          The occurrence and reduction of Giardia and Cryptosporidium in sewage and sludge from six Swedish treatment plants with Immunomagnetic separation/Immunofluorescens and Immunomagnetic separation in combination with a nested Polymerase Chain Reaction&nbsp

                    A. Hansen (Sweden)      


Beertour (optional)

Friday 24th  September


                    Waterborne transmission          

                    Chair: H. Smith (United Kingdom)


09.00          Giardia and Crypto: challenges to the water industry

                    G.J. Medema (The Netherlands)                 


09.30          Waterborne outbreaks of Cryptosporidium and Giardia infections

                    P.R. Hunter (United Kingdom)


10.00          Risk evaluation and the relationship between cyst/oocyst concentration in drinking water and waterborne disease

                    P. Wallis (Canada)                         


10.20          Genotype analysis for investigation of potential human-to-animal transmission of Cryptosporidiosis and Giardiasis through agricultural use of reclaimed wastewater

                    G. Di Giovanni (USA)     


10.40          Break


Drinking water treatment 2

                    Chair: P. Wallis (Canada)


11.00          Removal of Cryptosporidium and Giardia by water treatment processes

M. LeChevallier (USA)   


11.20          UV and Chemical Inactivation of Cryptosporidium and Giardia in Drinking Water Treatment - Compilation of Research at University of Alberta

                    S.A. Craik (Canada)      


11.40          Data analysis UK CryptoReg data

                    D. Drury            


12.00          Outlook on Cryptosporidium in drinking water

                    J. Colbourne   


12.40          Lunch with poster presentations


Methods & survival

                    Chair: M. LeChevallier (USA)


13.40          Detection of Giardia and Cryptosporidium in water

P. Karanis (Germany)    


14.00          Monitoring of foodstuffs for Cryptosporidium and Giardia                     

                    N. Cook (United Kingdom)           


14.20          Investigation of the survival of Cryptosporidium in environmental waters

                    P. Monis (Australia)        


14.40          Survival and development of Cryptosporidium parvum in water

                    A. Boxell (Australia)


15.00          Discussion


15.20          Break


15.50          Symposium conclusions


16.30          Closing ceremony with drinks


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Brief intro
Scientific Programme
Organising committee
On-line abstract submission
Hotel information
Travel & tourist information



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