Conventional light-ground illumination allows us to resolve the graticule with the 6mm lens and the sensor. At the centre, left extremity (appearing here like a grey rectangle) the transverse line spacing is 10 micrometers. The line spacing adjacent to this (centre) is at 100 micrometers, and the longer lines - left and right, marked by red crosses - are therefore one millimetre apart.
For reference, vertebrate cells are customarily 15 micrometers in diameter; cell aggregates often 40-50 micrometers in diameter. The separation of the 100 micrometer lines corresponds to that of a (coarse) human hair.
Go to next phase
Return to previous
phase